gbm7oj | Knowledge4COVID-19

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?:abstract	Coronavirus disease 2019 (COVID-19) has become pandemic since March 11, 2020. Thus, development and integration in clinics of fast and sensitive diagnostic tools are essential. The aim of the study is a development and evaluation of a one-step quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay (COVID-19 Amp) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection with an armored positive control and internal controls constructed from synthetic MS2-phage-based RNA particles. The COVID-19 Amp assay limit of detection was 103 copies/ml, the analytical specificity was 100%. A total of 109 biological samples were examined using COVID-19 Amp and World Health Organization (WHO)-based assay. Discordance in nine samples was observed (negative by the WHO-based assay) and discordant samples were retested as positive according to the results obtained from the Vector-PCRrv-2019-nCoV-RG assay. The developed COVID-19 Amp assay has high sensitivity and specificity, includes virus particles-based controls, provides the direct definition of the SARS-CoV-2 RdRp gene partial sequence, and is suitable for any hospital and laboratory equipped for RT-qPCR.
is ?:annotates of	<https://research.tib.eu/covid-19/entity/13gbm7oj_hasAnnotation_C0017337> <https://research.tib.eu/covid-19/entity/13gbm7oj_hasAnnotation_C0020517> <https://research.tib.eu/covid-19/entity/13gbm7oj_hasAnnotation_C0035668> <https://research.tib.eu/covid-19/entity/13gbm7oj_hasAnnotation_C0206390> <https://research.tib.eu/covid-19/entity/13gbm7oj_hasAnnotation_C1417126> <https://research.tib.eu/covid-19/entity/13gbm7oj_hasAnnotation_C5203676>
?:creator	<https://research.tib.eu/covid-19/entity/Goncharova%2C_Ekaterina_A%3B_Dedkov%2C_Vladimir_G%3B_Dolgova%2C_Anna_S%3B_Kassirov%2C_Ilia_S%3B_Safonova%2C_Marina_V%3B_Voytsekhovskaya%2C_Yana%3B_Totolian%2C_Areg_A>
?:journal	J._med._virol
?:license	unk
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
?:source	WHO
?:title	One-step quantitative RT-PCR assay with armored RNA controls for detection of SARS-CoV-2
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:who_covidence_id	#784292
?:year	2020

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