ltz4wyd

Property	Value
?:abstract	Infectious bronchitis (IB) is an economically important disease of poultry that also serve as model for the understanding of other coronaviruses associated diseases. IB is considered as a major challenge to the poultry industry worldwide as a result of its effect on egg production, weight gain as well as mortality. Different IBV genotypes continue to emerge, thus, the need for broad based vaccines to curb the disease. Based on bioinformatic data obtained in this study, sets of monovalent (either M41 or CR88) and bivalent DNA vaccines encoding the S1 glycoprotein from two different strains namely, M41 and CR88 were developed. The candidate vaccine was further encapsulated with a chitosan-saponin nanoparticle with the view to enhance its immunogenicity. Following in vitro characterization of the constructs, the vaccine candidates were tested in specific pathogen free (SPF) chickens. Analysis of humoral responses revealed a significant increase in anti-IBV antibody after immunization with the bivalent DNA plasmid (pBudCR88-S1/M41-S1). Likewise, cell mediated immune (CMI) response was significantly higher in vaccinated groups as compared to the unvaccinated chickens. Vaccinated chickens exhibited milder clinical signs as well as tracheal and kidney lesion scores following virus challenge as compared to the control groups. Additionally, encapsulation of the bivalent DNA vaccine with chitosan-saponin nanoparticles was found to improve protection against challenge with IBV strains M41 and CR88 as revealed by a significant reduction (p < 0.05) in oropharyngeal and cloacal virus sheddings following challenges with each of the two viruses. In contrast, monovalent IB-DNA vaccines were protective against homologous virus challenge. Moreover, nanoencasulation of the candidate vaccine was demostrated to abrogate the need for booster vaccination. In conclusion, this study demonstrates the immunogenic potentials of a bivalent IB-DNA vaccine and the use of chitosan-saponin nanoparticle to enhance its response. This development could serve as alternative strategy for the control of IB in poultry.
is ?:annotates of	<https://research.tib.eu/covid-19/entity/2ltz4wyd_hasAnnotation_C0042776> <https://research.tib.eu/covid-19/entity/2ltz4wyd_hasAnnotation_C0162633> <https://research.tib.eu/covid-19/entity/2ltz4wyd_hasAnnotation_C0206419> <https://research.tib.eu/covid-19/entity/2ltz4wyd_hasAnnotation_C0520481> <https://research.tib.eu/covid-19/entity/2ltz4wyd_hasAnnotation_C1334043> <https://research.tib.eu/covid-19/entity/2ltz4wyd_hasAnnotation_C3147110>
?:creator	<https://research.tib.eu/covid-19/entity/Bande%2C_Faruku%3B_Arshad%2C_Siti_Suri%3B_Bejo%2C_Mohd_Hair%3B_Omar%2C_Abdul_Rahman%3B_Moeini%2C_Hassan%3B_Khadkodae%2C_Saed%3B_Wei%2C_Tan_Sheau%3B_Keong%2C_Yeap_Swee%3B_Abba%2C_Yusuf%3B_Anka%2C_Ibrahim_Abubakar>
?:doi	<https://research.tib.eu/covid-19/entity/10.1016/j.micpath.2020.104560>
?:doi	10.1016/j.micpath.2020.104560
?:journal	Microb_Pathog
?:license	no-cc
?:pmc_json_files	document_parses/pmc_json/PMC7556284.xml.json
?:pmcid	<https://research.tib.eu/covid-19/entity/PMC7556284>
?:pmid	<https://research.tib.eu/covid-19/entity/33068733.0>
?:pmid	33068733.0
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
is ?:relation_isRelatedTo_publication of	<https://research.tib.eu/covid-19/entity/2ltz4wyd_HAS_C0032136_STIMULATES_C0003242>
?:source	Elsevier; Medline; PMC
?:title	Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:year	2020-10-14

Property

Value

?:abstract

Infectious bronchitis (IB) is an economically important disease of poultry that also serve as model for the understanding of other coronaviruses associated diseases. IB is considered as a major challenge to the poultry industry worldwide as a result of its effect on egg production, weight gain as well as mortality. Different IBV genotypes continue to emerge, thus, the need for broad based vaccines to curb the disease. Based on bioinformatic data obtained in this study, sets of monovalent (either M41 or CR88) and bivalent DNA vaccines encoding the S1 glycoprotein from two different strains namely, M41 and CR88 were developed. The candidate vaccine was further encapsulated with a chitosan-saponin nanoparticle with the view to enhance its immunogenicity. Following in vitro characterization of the constructs, the vaccine candidates were tested in specific pathogen free (SPF) chickens. Analysis of humoral responses revealed a significant increase in anti-IBV antibody after immunization with the bivalent DNA plasmid (pBudCR88-S1/M41-S1). Likewise, cell mediated immune (CMI) response was significantly higher in vaccinated groups as compared to the unvaccinated chickens. Vaccinated chickens exhibited milder clinical signs as well as tracheal and kidney lesion scores following virus challenge as compared to the control groups. Additionally, encapsulation of the bivalent DNA vaccine with chitosan-saponin nanoparticles was found to improve protection against challenge with IBV strains M41 and CR88 as revealed by a significant reduction (p < 0.05) in oropharyngeal and cloacal virus sheddings following challenges with each of the two viruses. In contrast, monovalent IB-DNA vaccines were protective against homologous virus challenge. Moreover, nanoencasulation of the candidate vaccine was demostrated to abrogate the need for booster vaccination. In conclusion, this study demonstrates the immunogenic potentials of a bivalent IB-DNA vaccine and the use of chitosan-saponin nanoparticle to enhance its response. This development could serve as alternative strategy for the control of IB in poultry.

is ?:annotates of