PropertyValue
?:abstract
  • Widespread, frequent testing is essential for curbing the ongoing COVID-19 pandemic. Because its simplicity makes it ideal for widely distributed, high throughput testing, RT-LAMP provides an attractive alternative to RT-qPCR. However, most RT-LAMP protocols require the purification of RNA, a complex and low-throughput bottleneck that has often been subject to reagent supply shortages. Here, we report an optimized RT-LAMP-based SARS-CoV-2 diagnostic protocol for saliva and swab samples. In the protocol we replace RNA purification with a simple sample preparation step using a widely available chelating agent, as well as optimize key protocol parameters. When tested on clinical swab and saliva samples, this assay achieves a limit of detection of 105 viral genomes per ml, with sensitivity close to 90% and specificity close to 100%, and takes 45 minutes from sample collection to result, making it well suited for a COVID-19 surveillance program.
is ?:annotates of
?:creator
?:doi
  • 10.1101/2020.11.19.20234948
?:doi
?:license
  • medrxiv
?:pdf_json_files
  • document_parses/pdf_json/f6bf970ce6c5ef2be369307b37f3d7afa6f1b1b4.json
?:publication_isRelatedTo_Disease
?:sha_id
?:source
  • MedRxiv; WHO
?:title
  • A simple direct RT-LAMP SARS-CoV-2 saliva diagnostic
?:type
?:year
  • 2020-11-22

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