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?:abstract
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In order to detect antibodies against foot-and-mouth disease virus (FMDV), the monoclonal antibody (MAb) 3D9was used as the capture antibody, and the HRP-labeled MAb 8E8 was used as the detection antibody After several conditions wereoptimized, the critical value of the detection was established A solid phase competition ELISA (SPCE) method for the detecting oftype O FMDV antibodies based on monoclonal antibodies Specific, sensitive, and reproducible tests were performed on this method The correlation between this method and the liquid phase blocking ELISA (LPBE) method and the virus neutralization test (VNT)was compared The results showed that the optimal dilution of MAb 3D9 was 1:25 000, the optimal dilution of inactivated type OFMDV antigen was 1:3, and the optimal dilution of MAb 8E8 was 1:5 000 When the serum was diluted at 1:32, the cut-off value of the assay was determined to be 45% The method was used to detect reference positive serum of type A FMDV, bovinecoronavirus, bovine rotavirus, porcine reproductive and respiratory syndrome virus, porcine circovirus 2, and classical swine fevervirus, respectively The test results were all negative and no cross-reaction occurred After testing, when the dilution of the positivestandard serum is 1:512, the method still has good sensitivity;the coefficient of variation of the intra-assay and inter-assay repeatedtests is less than 10%, indicating that the repeatability is better The correlation between this method and VNT and LPBE was 0 923and 0 896, respectively This study established a new method for the detection of domestic type O FMDV antibodies in China
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