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  • Catalysis of the reaction: 2\'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP ribose 1\'\',2\'\'-phosphate + nicotinamide + H2O. This reaction is the transfer of the splice junction 2-phosphate from ligated tRNA to NAD+ to produce ADP-ribose 1\'-2\' cyclic phosphate. [EC:2.7.1.160, PMID:9148937]
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