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  • The process of coupling phenylalanine to phenylalanyl-tRNA, catalyzed by phenylalanyl-tRNA synthetase. The phenylalanyl-tRNA synthetase is a class-II synthetase. However, unlike other class II enzymes, The activated amino acid is transferred to the 2\'-OH group of a phenylalanine-accepting tRNA. The 2\'-O-aminoacyl-tRNA will ultimately migrate to the 3\' position via transesterification. [GOC:mcc, ISBN:0716730510]
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