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  • Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing thymine at position U54 of a transfer RNA. This occurs in most Gram-negative bacteria, some archae, and eukaryotes. [GOC:hjd, ISBN:1555811337]
  • Catalysis of the transfer of a methyl group from a donor to the C5 atom of the uridine residue at position 54 in a tRNA molecule. [ISBN:1555811337]
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