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  • Catalysis of the hydrolysis of a 5\' tyrosyl-RNA phosphodiester bond between a protein and RNA. In picornaviruses, this covalent bond connects VPg, a viral-encoded protein essential for RNA replication, to the 5\' end of all nascent picornavirus genomes; it is cleaved from viral RNA prior to its engaging in protein synthesis. [GOC:bf, GOC:sp, PMID:21408223, PMID:22908287]
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