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Genetic disorders pose as a significant threat to the well-being of human life in this century, as more and more diseases identified are related to mutations presented in patients’ genome [ ]there are limited target sites due to requirement of PAM sequence for DNA cleavage, such as NGG for Cas9 [ ]the safety and ethical issues of the CRISPR applications need re-evaluations along with the discovery of more details about its capabilities [ ]occurring stem cells in adult human tissues are another kinds of stem cells that only have restricted potency (Blau and Daley 2019)
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Combined application of CRISPR-Cas and stem cells for clinical and basic research
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