flyjmamy | Knowledge4COVID-19

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?:abstract	INTRODUCTION: Various viruses including a novel coronavirus (SARS-CoV-2) can infect the kidney. When viruses invade the glomeruli from the bloodstream, glomerular endothelial cells (GECs) initiate the innate immune reactions. We investigated the expression of interferon (IFN)-induced protein with tetratricopeptide repeats (IFIT) 1/2/3, antiviral molecules, in human GECs treated with a toll-like receptor (TLR) 3 agonist. Role of IFIT1/2/3 in the expression of C-X-C motif chemokine ligand 10 (CXCL10) was also examined. METHODS: Human GECs were cultured and stimulated with polyinosinic-polycytidylic acid (poly IC), a synthetic TLR3 agonist. Real-time qPCR, Western blotting, and ELISA were used to examine the expression of IFIT1/2/3, IFN-ß, and CXCL10. RNA interference against IFN-ß or IFIT1/2/3 was also performed. RESULTS: Expression of IFIT1/2/3 and CXCL10 was induced by poly IC in GECs. The inductions were inhibited by RNA interfering of IFN-ß. Knockdown of IFIT1/2/3 decreased the CXCL10 expression. Knockdown of IFIT3 decreased the expression of IFIT1 and IFIT2 proteins. CONCLUSION: IFIT1/2/3 and CXCL10 were induced by poly IC via IFN-ß in GECs. IFIT1/2/3 may increase the expression of CXCL10 which induces lymphocyte chemotaxis and may inhibit the replication of infected viruses. These molecules may play a role in GEC innate immune reactions in response to viruses.
is ?:annotates of	<https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C0035668> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C0042776> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C1367714> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C1415892> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C1415894> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C1415895> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C2987634> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C4505239> <https://research.tib.eu/covid-19/entity/flyjmamy_hasAnnotation_C5203676>
?:creator	<https://research.tib.eu/covid-19/entity/Imaizumi%2C_Tadaatsu%3B_Hashimoto%2C_Shun%3B_Sato%2C_Riko%3B_Umetsu%2C_Hidenori%3B_Aizawa%2C_Tomomi%3B_Watanabe%2C_Shojiro%3B_Kawaguchi%2C_Shogo%3B_Matsumiya%2C_Tomoh%3B_Seya%2C_Kazuhiko%3B_Ding%2C_Jiangli%3B_Tanaka%2C_Hiroshi>
?:journal	Kidney_Blood_Press_Res
?:license	unk
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
is ?:relation_isRelatedTo_publication of	<https://research.tib.eu/covid-19/entity/flyjmamy_HAS_C0032414_INTERACTS_WITH_C0015980> <https://research.tib.eu/covid-19/entity/flyjmamy_HAS_C0032414_STIMULATES_C0015980> <https://research.tib.eu/covid-19/entity/flyjmamy_HAS_C0032414_STIMULATES_C1415892> <https://research.tib.eu/covid-19/entity/flyjmamy_HAS_C0035668_DISRUPTS_C1182791> <https://research.tib.eu/covid-19/entity/flyjmamy_HAS_C1367714_STIMULATES_C0032414> <https://research.tib.eu/covid-19/entity/flyjmamy_HAS_C1415895_INHIBITS_C1415892> <https://research.tib.eu/covid-19/entity/flyjmamy_HAS_C1415895_INHIBITS_C1415894>
?:source	WHO
?:title	IFIT Proteins Are Involved in CXCL10 Expression in Human Glomerular Endothelial Cells Treated with a Toll-Like Receptor 3 Agonist
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:who_covidence_id	#978847
?:year	2020

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