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Standard diagnostic methods of Coronavirus Disease 2019 (COVID-19) rely on RT-qPCR technique which have limited point-of-care test (POCT) potential due to necessity of dedicated equipment and specialized personnel LAMP, an isothermal nucleic acid amplification test (NAAT), is a promising technique that may substitute RT-qPCR for POCT of genomic materials Here, we provide a protocol to perform reverse transcription LAMP targeting SARS-CoV-2 We adopted both real-time fluorescence detection and end-point colorimetric detection approaches Our protocol would be useful for screening diagnosis of COVID-19 and be a baseline for development of improved POCT NAAT
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Colorimetric RT-LAMP Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
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