n82v8fp2

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?:abstract	STUDY QUESTION: Is SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE 2) expressed in the human endometrium during the menstrual cycle, and does it participate in endometrial decidualization? SUMMARY ANSWER: ACE2 protein is highly expressed in human endometrial stromal cells during the secretory phase and is essential for human endometrial stromal cell decidualization WHAT IS KNOWN ALREADY: ACE2 is expressed in numerous human tissues including the lungs, heart, intestine, kidneys and placenta ACE2 is also the receptor by which SARS-CoV-2 enters human cells STUDY DESIGN SIZE DURATION: Proliferative (n = 9) and secretory (n = 6) phase endometrium biopsies from healthy reproductive-age women and primary human endometrial stromal cells from proliferative phase endometrium were used in the study PARTICIPANTS/MATERIALS SETTING METHODS: ACE2 expression and localization were examined by qRT-PCR, Western blot, and immunofluorescence in both human endometrial samples and mouse uterine tissue The effect of ACE2 knockdown on morphological and molecular changes of human endometrial stromal cell decidualization were assessed Ovariectomized mice were treated with estrogen or progesterone to determine the effects of these hormones on ACE2 expression MAIN RESULTS AND THE ROLE OF CHANCE: In human tissue, ACE2 protein is expressed in both endometrial epithelial and stromal cells in the proliferative phase of the menstrual cycle, and expression increases in stromal cells in the secretory phase The ACE2 mRNA ( P < 0 0001) and protein abundance increased during primary human endometrial stromal cell (HESC) decidualization HESCs transfected with ACE2 -targeting siRNA were less able to decidualize than controls, as evidenced by a lack of morphology change and lower expression of the decidualization markers PRL and IGFBP1 ( P < 0 05) In mice during pregnancy, ACE2 protein was expressed in uterine epithelial and stromal cells increased through day six of pregnancy Finally, progesterone induced expression of Ace2 mRNA in mouse uteri more than vehicle or estrogen ( P < 0 05) Large scale data: N/a LIMITATIONS REASONS FOR CAUTION: Experiments assessing the function of ACE2 in human endometrial stromal cell decidualization were in vitro Whether SARS-CoV-2 can enter human endometrial stromal cells and affect decidualization have not been assessed WIDER IMPLICATIONS OF THE FINDINGS: Expression of ACE2 in the endometrium allow SARS-CoV-2 to enter endometrial epithelial and stromal cells, which could impair in vivo decidualization, embryo implantation, and placentation If so, women with COVID-19 may be at increased risk of early pregnancy loss STUDY FUNDINGS/COMPETING INTERESTS: This study was supported by National Institutes of Health / National Institute of Child Health and Human Development grants R01HD065435 and R00HD080742 to RK and Washington University School of Medicine start-up funds to RK The authors declare that they have no conflicts of interest The coronavirus disease 2019 (COVID-19) first appeared in December 2019 and rapidly spread throughout the world. The SARS-CoV-2 virus enters the host cells by binding to the angiotensin-converting enzyme (ACE2). Although much of the focus is on respiratory symptoms, recent reports suggest that SARS-CoV-2 can cause pregnancy complications such as pre-term birth and miscarriages; and women with COVID-19 had maternal vascular malperfusion and decidual arteriopathy in their placentas. Here, we report that ACE2 protein is expressed in both endometrial epithelial and stromal cells in the proliferative phase of the menstrual cycle, and expression increases in stromal cells in the secretory phase. The ACE2 mRNA and protein abundance increased during primary human endometrial stromal cell (HESC) decidualization. Further, HESCs transfected with ACE2-targeting siRNA impaired the full decidualization response, as evidenced by a lack of morphology change and lower expression of the decidualization markers PRL and IGFBP1. Additionally, in mice during pregnancy, ACE2 protein was expressed in uterine epithelial, and stromal cells increased through day six of pregnancy. Finally, progesterone induced Ace2 mRNA expression in mouse uteri more than vehicle or estrogen. These data establish a role for ACE2 in endometrial physiology, suggesting that SARS-CoV-2 may be able to enter endometrial stromal cells and elicit pathological manifestations in women with COVID-19 including an increased risk of early pregnancy loss.
?:creator	<https://research.tib.eu/covid-19/entity/Chadchan%2C_S._B.%3B_Maurya%2C_V._K.%3B_Popli%2C_P.%3B_Kommagani%2C_R.> <https://research.tib.eu/covid-19/entity/Chadchan%2C_Sangappa_B%3B_Popli%2C_Pooja%3B_Maurya%2C_Vineet_K%3B_Kommagani%2C_Ramakrishna>
?:journal	BioRxiv_:_the_Preprint_Server_for_Biology Biol._reprod
?:license	unk
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
?:source	WHO
?:title	The SARS-CoV-2 receptor, Angiotensin converting enzyme 2 (ACE2) is required for human endometrial stromal cell decidualization
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:who_covidence_id	#900739 #965922
?:year	2020

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?:abstract

STUDY QUESTION: Is SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE 2) expressed in the human endometrium during the menstrual cycle, and does it participate in endometrial decidualization? SUMMARY ANSWER: ACE2 protein is highly expressed in human endometrial stromal cells during the secretory phase and is essential for human endometrial stromal cell decidualization WHAT IS KNOWN ALREADY: ACE2 is expressed in numerous human tissues including the lungs, heart, intestine, kidneys and placenta ACE2 is also the receptor by which SARS-CoV-2 enters human cells STUDY DESIGN SIZE DURATION: Proliferative (n = 9) and secretory (n = 6) phase endometrium biopsies from healthy reproductive-age women and primary human endometrial stromal cells from proliferative phase endometrium were used in the study PARTICIPANTS/MATERIALS SETTING METHODS: ACE2 expression and localization were examined by qRT-PCR, Western blot, and immunofluorescence in both human endometrial samples and mouse uterine tissue The effect of ACE2 knockdown on morphological and molecular changes of human endometrial stromal cell decidualization were assessed Ovariectomized mice were treated with estrogen or progesterone to determine the effects of these hormones on ACE2 expression MAIN RESULTS AND THE ROLE OF CHANCE: In human tissue, ACE2 protein is expressed in both endometrial epithelial and stromal cells in the proliferative phase of the menstrual cycle, and expression increases in stromal cells in the secretory phase The ACE2 mRNA ( P < 0 0001) and protein abundance increased during primary human endometrial stromal cell (HESC) decidualization HESCs transfected with ACE2 -targeting siRNA were less able to decidualize than controls, as evidenced by a lack of morphology change and lower expression of the decidualization markers PRL and IGFBP1 ( P < 0 05) In mice during pregnancy, ACE2 protein was expressed in uterine epithelial and stromal cells increased through day six of pregnancy Finally, progesterone induced expression of Ace2 mRNA in mouse uteri more than vehicle or estrogen ( P < 0 05) Large scale data: N/a LIMITATIONS REASONS FOR CAUTION: Experiments assessing the function of ACE2 in human endometrial stromal cell decidualization were in vitro Whether SARS-CoV-2 can enter human endometrial stromal cells and affect decidualization have not been assessed WIDER IMPLICATIONS OF THE FINDINGS: Expression of ACE2 in the endometrium allow SARS-CoV-2 to enter endometrial epithelial and stromal cells, which could impair in vivo decidualization, embryo implantation, and placentation If so, women with COVID-19 may be at increased risk of early pregnancy loss STUDY FUNDINGS/COMPETING INTERESTS: This study was supported by National Institutes of Health / National Institute of Child Health and Human Development grants R01HD065435 and R00HD080742 to RK and Washington University School of Medicine start-up funds to RK The authors declare that they have no conflicts of interest
The coronavirus disease 2019 (COVID-19) first appeared in December 2019 and rapidly spread throughout the world. The SARS-CoV-2 virus enters the host cells by binding to the angiotensin-converting enzyme (ACE2). Although much of the focus is on respiratory symptoms, recent reports suggest that SARS-CoV-2 can cause pregnancy complications such as pre-term birth and miscarriages; and women with COVID-19 had maternal vascular malperfusion and decidual arteriopathy in their placentas. Here, we report that ACE2 protein is expressed in both endometrial epithelial and stromal cells in the proliferative phase of the menstrual cycle, and expression increases in stromal cells in the secretory phase. The ACE2 mRNA and protein abundance increased during primary human endometrial stromal cell (HESC) decidualization. Further, HESCs transfected with ACE2-targeting siRNA impaired the full decidualization response, as evidenced by a lack of morphology change and lower expression of the decidualization markers PRL and IGFBP1. Additionally, in mice during pregnancy, ACE2 protein was expressed in uterine epithelial, and stromal cells increased through day six of pregnancy. Finally, progesterone induced Ace2 mRNA expression in mouse uteri more than vehicle or estrogen. These data establish a role for ACE2 in endometrial physiology, suggesting that SARS-CoV-2 may be able to enter endometrial stromal cells and elicit pathological manifestations in women with COVID-19 including an increased risk of early pregnancy loss.

?:creator