PropertyValue
?:abstract
  • The newly identified 2019-nCoV (SARS-CoV-2) RNA virus has caused over 80,000 laboratory-confirmed human infections in China (as of Mar 1st, 2020), and is now becoming a global epidemic issue As a result, highly sensitive, reliable and field-deployable methods to detect 2019-nCoV that can be developed in a shortest possible time are urgently needed In this work, we introduce whole-course encapsulated and ultrasensitive field detection methods against 2019-nCoV gene We applied isothermal exponential amplification methods via reverse transcription plus subsequent enzymatic recombinase amplification (ERA), a modified recombinase polymerase amplification (RPA), to amplify trace level of 2019-nCoV RNA We designed both exo FRET probe and nfo affinity probe for easy detection of the amplified nucleic acids These methods can be developed within a few days and as low as 0 32 aM (i e 0 32×10-18 M) of RNA can be reliably detected br
is ?:annotates of
?:creator
?:license
  • unk
?:publication_isRelatedTo_Disease
is ?:relation_isRelatedTo_publication of
?:source
  • WHO
?:title
  • Ultrasensitive and Whole-Course Encapsulated Field Detection of 2019-nCoV Gene Applying Exponential Amplification from RNA Combined with Chemical Probes
?:type
?:who_covidence_id
  • #393
?:year
  • 2020

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