wh15qo

Property	Value
?:abstract	RESEARCH QUESTION: : What are the risks associated with cryopreserved semen collected during and after the coronavirus disease 2019 (COVID-19) pandemic wave in Wuhan, China? DESIGN: : We conducted a retrospective cohort study involving young adult men who were qualified sperm donors at the Hunan Province Human Sperm Bank (China) during the pandemic wave (January 1, 2020, to January 30, 2020) and after the wave and return to work (April 7, 2020, to May 30, 2020). One hundred paired semen and blood specimens from 100 donors were included, 50 paired semen and blood samples collected during the pandemic from 50 donors, and 50 semen and blood samples from another 50 donors collected upon work resumption when COVID-19 infections in the population were very low. Considering the low viral load in both the specimens, we used one-step single-tube nested quantitative real-time PCR (OSN-qRT-PCR) for SARS-CoV-2 detection. Moreover, to control the unacceptable risk of false-negative results, a second round of screening was performed with pooled RNA from negative semen samples using crystal digital PCR (cd-PCR). Naso-oropharyngeal swabs were not evaluated during the pandemic peak, but were negative for the 50 donors who collected semen after the COVID-19 wave of infections. RESULTS: : For individual blood and semen samples, the target genes, namely nucleocapsid protein (N) and open reading frame (ORF-1ab) genes, tested negative in all of the 100 paired samples. Further, as per cd-PCR results, there were >20,000 droplets per well in the RNA for each combined sample and no positive droplets were present for either of the aforementioned target genes. A total of 100 paired semen and blood samples from these two groups tested negative for SARS-CoV-2. CONCLUSIONS: : Cryopreserved semen at the Hunan Province Human Sperm Bank during and after the coronavirus disease 2019 (COVID-19) pandemic wave was free of SARS-CoV-2, and was judged safe for external use in the future.Utmost precaution should be exercised by all human sperm banks, and the use of highly secure devices and segregated cryovessels is encouraged.
is ?:annotates of	<https://research.tib.eu/covid-19/entity/19wh15qo_hasAnnotation_C0017337> <https://research.tib.eu/covid-19/entity/19wh15qo_hasAnnotation_C0035668> <https://research.tib.eu/covid-19/entity/19wh15qo_hasAnnotation_C0079941> <https://research.tib.eu/covid-19/entity/19wh15qo_hasAnnotation_C1332838> <https://research.tib.eu/covid-19/entity/19wh15qo_hasAnnotation_C1421479> <https://research.tib.eu/covid-19/entity/19wh15qo_hasAnnotation_C3714514> <https://research.tib.eu/covid-19/entity/19wh15qo_hasAnnotation_C5203676>
?:creator	<https://research.tib.eu/covid-19/entity/Huang%2C_Chuan%3B_Zhou%2C_Shuai-Feng%3B_Gao%2C_Li-Dong%3B_Li%2C_Shi-Kang%3B_Cheng%2C_Yu%3B_Zhou%2C_Wen-Jun%3B_Huang%2C_Zeng-Hui%3B_Ji%2C_Xi-Ren%3B_Fan%2C_Li-Qing%3B_Zhang%2C_Fan%3B_Chen%2C_Zi-Wei%3B_Zhan%2C_Zhi-Fei%3B_Hu%2C_Shi-Xiong%3B_Wang%2C_Shi-Ping%3B_Ma%2C_Xue-Jun%3B_Zhu%2C_Wen-Bing>
?:doi	<https://research.tib.eu/covid-19/entity/10.1016/j.rbmo.2020.11.015>
?:doi	10.1016/j.rbmo.2020.11.015
?:journal	Reprod_Biomed_Online
?:license	no-cc
?:pdf_json_files	document_parses/pdf_json/bf0b07c4ae026187ac0f169fefc21fca0d58a2f8.json
?:pmc_json_files	document_parses/pmc_json/PMC7713547.xml.json
?:pmcid	<https://research.tib.eu/covid-19/entity/PMC7713547>
?:pmid	<https://research.tib.eu/covid-19/entity/33384268.0>
?:pmid	33384268.0
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
?:sha_id	<https://research.tib.eu/covid-19/entity/bf0b07c4ae026187ac0f169fefc21fca0d58a2f8>
?:source	Elsevier; Medline; PMC
?:title	Risks associated with cryopreserved semen in a human sperm bank during and after the COVID-19 pandemic
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:year	2020-12-03

Property

Value

?:abstract

RESEARCH QUESTION: : What are the risks associated with cryopreserved semen collected during and after the coronavirus disease 2019 (COVID-19) pandemic wave in Wuhan, China? DESIGN: : We conducted a retrospective cohort study involving young adult men who were qualified sperm donors at the Hunan Province Human Sperm Bank (China) during the pandemic wave (January 1, 2020, to January 30, 2020) and after the wave and return to work (April 7, 2020, to May 30, 2020). One hundred paired semen and blood specimens from 100 donors were included, 50 paired semen and blood samples collected during the pandemic from 50 donors, and 50 semen and blood samples from another 50 donors collected upon work resumption when COVID-19 infections in the population were very low. Considering the low viral load in both the specimens, we used one-step single-tube nested quantitative real-time PCR (OSN-qRT-PCR) for SARS-CoV-2 detection. Moreover, to control the unacceptable risk of false-negative results, a second round of screening was performed with pooled RNA from negative semen samples using crystal digital PCR (cd-PCR). Naso-oropharyngeal swabs were not evaluated during the pandemic peak, but were negative for the 50 donors who collected semen after the COVID-19 wave of infections. RESULTS: : For individual blood and semen samples, the target genes, namely nucleocapsid protein (N) and open reading frame (ORF-1ab) genes, tested negative in all of the 100 paired samples. Further, as per cd-PCR results, there were >20,000 droplets per well in the RNA for each combined sample and no positive droplets were present for either of the aforementioned target genes. A total of 100 paired semen and blood samples from these two groups tested negative for SARS-CoV-2. CONCLUSIONS: : Cryopreserved semen at the Hunan Province Human Sperm Bank during and after the coronavirus disease 2019 (COVID-19) pandemic wave was free of SARS-CoV-2, and was judged safe for external use in the future.Utmost precaution should be exercised by all human sperm banks, and the use of highly secure devices and segregated cryovessels is encouraged.

is ?:annotates of