PropertyValue
?:abstract
  • The main protease, M(pro), of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M(pro) function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.
is ?:annotates of
?:creator
?:doi
?:doi
  • 10.1101/2020.11.09.375139
?:journal
  • bioRxiv
?:license
  • cc-by-nd
?:pdf_json_files
  • document_parses/pdf_json/6029e4120ebe78aefaf6bba876d74d07bfd2d42f.json; document_parses/pdf_json/c8e83cdddd1b2cc85e318f6e312383026abd223f.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7668733.xml.json
?:pmcid
?:pmid
?:pmid
  • 33200129.0
?:publication_isRelatedTo_Disease
?:sha_id
?:source
  • BioRxiv; Medline; PMC; WHO
?:title
  • Gain-of-function assay for SARS-CoV-2 M(pro) inhibition in living cells
?:type
?:year
  • 2020-11-09

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