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?:abstract
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The main protease, M(pro), of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M(pro) function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.
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?:creator
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?:doi
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?:doi
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10.1101/2020.11.09.375139
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?:journal
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?:license
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?:pdf_json_files
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document_parses/pdf_json/6029e4120ebe78aefaf6bba876d74d07bfd2d42f.json; document_parses/pdf_json/c8e83cdddd1b2cc85e318f6e312383026abd223f.json
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document_parses/pmc_json/PMC7668733.xml.json
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?:pmid
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?:sha_id
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?:source
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BioRxiv; Medline; PMC; WHO
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?:title
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Gain-of-function assay for SARS-CoV-2 M(pro) inhibition in living cells
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?:year
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