PropertyValue
?:abstract
  • The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, we found that pooling of 5 samples showed that CT variations were in the range of 1.0-4,5 units, with less likelihood of a false negative result. Results of the sample without nucleic acid ex-traction, was unsatisfactory, with a significant increase in CT values, and thus for risk of a false negative result. In conclusion, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries.
?:creator
?:doi
?:doi
  • 10.4067/s0716-10182020000300276
?:journal
  • Revista_chilena_de_infectologia_:_organo_oficial_de_la_Sociedad_Chilena_de_Infectologia
?:license
  • cc-by-nc
?:pmid
?:pmid
  • 32853320
?:publication_isRelatedTo_Disease
?:source
  • Medline
?:title
  • Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing.
?:type
?:year
  • 2020-06-01

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