PropertyValue
?:abstract
  • In resource-limited countries, early detection of novel pathogens is often challenging, due to financial and technical constraints. This study reports the efficacy of family-wide polymerase chain reaction (PCR) in screening, detecting, and identifying initial cases of the novel SARS-CoV-2 in Thailand. Respiratory secretions were collected from suspected individuals traveling from Wuhan, China to Thailand at the beginning of January 2020. Family-wide PCR assays yielded positive results for coronavirus in one traveler within 12 h on January 8, 2020. Nucleotide sequences (290 bp) showed 100% similarity to SARS-CoV-2. The whole genome sequence was further characterized by Next Generation Sequencing (NGS) for confirmation. Combining family-wide PCR, as a rapid screening tool, with NGS, for full genome characterization, could facilitate early detection and confirmation of a novel pathogen and enable early containment of a disease outbreak.
is ?:annotates of
?:creator
?:doi
  • 10.3389/fpubh.2020.555013
?:doi
?:journal
  • Front_Public_Health
?:license
  • cc-by
?:pdf_json_files
  • document_parses/pdf_json/7265f063ed3eaaddcb804ff720cd89cc962a9299.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7579402.xml.json
?:pmcid
?:pmid
?:pmid
  • 33134237.0
?:publication_isRelatedTo_Disease
?:sha_id
?:source
  • Medline; PMC
?:title
  • Identification of a Novel Pathogen Using Family-Wide PCR: Initial Confirmation of COVID-19 in Thailand
?:type
?:year
  • 2020-10-07

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