PropertyValue
?:abstract
  • Abstract The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, we found that pooling of 5 samples showed that CT variations were in the range of 1.0-4,5 units, with less likelihood of a false negative result. Results of the sample without nucleic acid ex-traction, was unsatisfactory, with a significant increase in CT values, and thus for risk of a false negative result. In conclusion, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries.
is ?:annotates of
?:creator
?:journal
  • Rev._chil._infectol
?:license
  • unk
?:publication_isRelatedTo_Disease
?:source
  • WHO
?:title
  • Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing/ Optimización de la detección de SARS-CoV-2 mediante el análisis de muestras agrupadas
?:type
?:who_covidence_id
  • #724396
  • #734284
  • #887641
?:year
  • 2020

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