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?:abstract
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OBJECTIVE: SARS-CoV-2 is sweeping the world since the end of 2019. The titer change of antibodies against SARS-CoV-2 needs to be further clarified, the clinical and preventive value of antibodies still needs to be further investigated. METHOD: An ELISA was established by coating with SARS-CoV-2 recombinant spike protein and used to detect serum IgM and IgG antibodies against SARS-CoV-2 in COVID-19 patients to evaluate the pattern of changes of antibodies. RESULTS: The specificity of the ELISA for detection SARS-CoV-2 IgM and IgG were 96% (144/150) and 100% (150/150), respectively. The sensitivity of ELISA was 100% (150/150) for IgM, and 99.3% (149/150) for IgG. SARS-CoV-2-SP-IgM and SP-IgG antibodies could be detected on day 1 of hospitalization in 12.5% patients, and SP-IgM began to decrease after reaching its peak at around 22-28 days, and become negative at month 3 in 30% patients and negative at month 7 in 79% of these patients after onset; IgG reached its peak around day 22-28 and kept at a high level within the longest observation period for 4 months, it dropped very sharply at 7 months. The positive rates of SP-IgM and SP-IgG were higher than those of RT-qPCR on day 7 and 4. CONCLUSION: The established indirect ELISA has good specificity and sensitivity. IgM and IgG against SARS-CoV-2 appeared almost simultaneously in the early stage, and the level of IgG antibodies couldn\'t maintain a high plateau in the observation period of 7 months. Our data will help develop the diagnosis and vaccine of SARS-CoV-2. This article is protected by copyright. All rights reserved.
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