ohxj27a

Property	Value
?:abstract	OBJECTIVES: Due to a substantial proportion of asymptomatic and mild courses, many SARS-CoV-2 infections remain unreported. Therefore, assessment of seroprevalence may detect the real burden of disease. We aimed at determining and characterizing the rate of SARS-CoV-2 infections and the resulting seroprevalence in a defined population. The primary objective of the study was to assess SARS-CoV-2 antibody seroprevalence using six different IgG detecting immunoassays. Secondary objectives of the study were: i.) to determine potential risk factors for symptomatic vs. asymptomatic Covid19 courses; ii.) to investigate the rate of virus RNA-persistence. METHODS: CoNAN is a population-based cohort study performed in the community Neustadt am Rennsteig, Germany that was quarantined from March 22(nd) to April 5(th) after six SARS-CoV-2 cases were detected in the villages’ population. The SARS-CoV-2 outbreak compromised 51 cases and 3 deaths. The CoNAN study was performed from May 13th to May 22nd 2020 six weeks after a SARS-CoV-2 outbreak. RESULTS: We enrolled a total of 626 participants (71% of the community population) for PCR- and antibody testing in the study. All actual SARS-CoV-2 PCR tests were negative. Fifty-two out of 620 (8.4%) participants had antibodies against SARS-CoV-2 in at least two different assays. There were 38 participants with previously PCR-confirmed SARS-CoV-2 infection. Of those, only 19 (50%) displayed anti-SARS-CoV-2 antibodies. We also show that antibody positive participants with symptoms compatible with a respiratory tract infection had significantly higher antibody levels then asymptomatic participants (EU-assay: Median 2.9 vs. 7.2 IgG-index, p=0.002; DS-assay: Median 45.2 vs. 143 AU/mL, p=0.002). Persisting viral replication was not detected. CONCLUSIONS: Our data question the relevance and reliability of IgG antibody testing to detect past SARS-CoV-2 infections six weeks after an outbreak. We conclude that assessing immunity for SARS-CoV-2 infection should not only rely on antibody tests.
is ?:annotates of	<https://research.tib.eu/covid-19/entity/6ohxj27a_hasAnnotation_C0027627> <https://research.tib.eu/covid-19/entity/6ohxj27a_hasAnnotation_C0035691> <https://research.tib.eu/covid-19/entity/6ohxj27a_hasAnnotation_C1417168> <https://research.tib.eu/covid-19/entity/6ohxj27a_hasAnnotation_C1457887> <https://research.tib.eu/covid-19/entity/6ohxj27a_hasAnnotation_C5203676>
?:creator	<https://research.tib.eu/covid-19/entity/Weis%2C_Sebastian%3B_Scherag%2C_Andr%C3%A9%3B_Baier%2C_Michael%3B_Kiehntopf%2C_Michael%3B_Kamradt%2C_Thomas%3B_Kolanos%2C_Steffi%3B_Ankert%2C_Juliane%3B_Gl%C3%B6ckner%2C_Stefan%3B_Makarewicz%2C_Oliwia%3B_Hagel%2C_Stefan%3B_Bahrs%2C_Christina%3B_Kimmig%2C_Aurelia%3B_Proquitt%C3%A9%2C_Hans%3B_Guerra%2C_Joel%3B_Rimek%2C_Dagmar%3B_L%C3%B6ffler%2C_Bettina%3B_Pletz%2C_Mathias_W.>
?:doi	10.1016/j.cmi.2020.11.009
?:doi	<https://research.tib.eu/covid-19/entity/10.1016/j.cmi.2020.11.009>
?:journal	Clin_Microbiol_Infect
?:license	no-cc
?:pdf_json_files	document_parses/pdf_json/700a21efebcb75dd3e5b5cfbf3e91a90389b42fd.json
?:pmc_json_files	document_parses/pmc_json/PMC7677041.xml.json
?:pmcid	<https://research.tib.eu/covid-19/entity/PMC7677041>
?:pmid	<https://research.tib.eu/covid-19/entity/33221432.0>
?:pmid	33221432.0
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
?:sha_id	<https://research.tib.eu/covid-19/entity/700a21efebcb75dd3e5b5cfbf3e91a90389b42fd>
?:source	Elsevier; Medline; PMC
?:title	Antibody response using six different serological assays in a completely PCR-tested community after a COVID-19 outbreak - The CoNAN study
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:year	2020-11-20

Property

Value

?:abstract

OBJECTIVES: Due to a substantial proportion of asymptomatic and mild courses, many SARS-CoV-2 infections remain unreported. Therefore, assessment of seroprevalence may detect the real burden of disease. We aimed at determining and characterizing the rate of SARS-CoV-2 infections and the resulting seroprevalence in a defined population. The primary objective of the study was to assess SARS-CoV-2 antibody seroprevalence using six different IgG detecting immunoassays. Secondary objectives of the study were: i.) to determine potential risk factors for symptomatic vs. asymptomatic Covid19 courses; ii.) to investigate the rate of virus RNA-persistence. METHODS: CoNAN is a population-based cohort study performed in the community Neustadt am Rennsteig, Germany that was quarantined from March 22(nd) to April 5(th) after six SARS-CoV-2 cases were detected in the villages’ population. The SARS-CoV-2 outbreak compromised 51 cases and 3 deaths. The CoNAN study was performed from May 13th to May 22nd 2020 six weeks after a SARS-CoV-2 outbreak. RESULTS: We enrolled a total of 626 participants (71% of the community population) for PCR- and antibody testing in the study. All actual SARS-CoV-2 PCR tests were negative. Fifty-two out of 620 (8.4%) participants had antibodies against SARS-CoV-2 in at least two different assays. There were 38 participants with previously PCR-confirmed SARS-CoV-2 infection. Of those, only 19 (50%) displayed anti-SARS-CoV-2 antibodies. We also show that antibody positive participants with symptoms compatible with a respiratory tract infection had significantly higher antibody levels then asymptomatic participants (EU-assay: Median 2.9 vs. 7.2 IgG-index, p=0.002; DS-assay: Median 45.2 vs. 143 AU/mL, p=0.002). Persisting viral replication was not detected. CONCLUSIONS: Our data question the relevance and reliability of IgG antibody testing to detect past SARS-CoV-2 infections six weeks after an outbreak. We conclude that assessing immunity for SARS-CoV-2 infection should not only rely on antibody tests.

is ?:annotates of