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Recent advancements in paired B cell receptor (BCR) sequencing technologies have accelerated the development of simpler, higher-throughput pipelines for generating native antibody heavy and light chain pairs used to elucidate novel antibodies and provide insights into antibody response against pathogenic targets. These technologies involve single-cell isolation, using either single wells or emulsified droplets to maintain physical separation of individual cells, followed by sequencing. The development of novel single well and emulsion-based workflows address key challenges by improving throughput of single-cell analyses, reducing method complexity, and integrating functional assays into existing workflows. Enabled by paired BCR sequencing, functional characterization of pathogen-specific antibodies reveals immunological insights beyond bulk sequencing.
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10.1016/j.coisb.2020.10.008
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document_parses/pmc_json/PMC7568503.xml.json
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Beyond Bulk Single-chain Sequencing – Getting at the Whole Receptor
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