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?:abstract
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Objective: Previous work by our group (1) showed that the rate of chromosomal mosaicism decreases with maternal age However, the types of chromosomes involved, as well as the types of chromosomal mosaicism in individual embryos, have not yet been examined Our objective was to determine whether maternal age was associated with the rate of sex and autosomal chromosome mosaicism and the rates of various types of mosaicism Design: Retrospective cohort study of all blastocysts that underwent trophectoderm biopsy for preimplantation genetic testing for aneuploidy (PGT-A) from 1/2015 to 12/2018 at our center Materials and Methods: All patients with blastocysts that underwent trophectoderm biopsy for PGT-A via Next Generation Sequencing with ≥1 chromosome in the mosaic range (20-80%) were included The primary outcomes were: 1) the rate of sex and autosomal chromosome mosaicism and 2) rates of segmental mosaicism, full chromosome mosaicism and complex (≥3 mosaic chromosomes) stratified by maternal age Statistical analyses included Kruskal-Wallis (KW) and linear regression (LR) to control for paternal age, with p<0 05 considered significant Results: 1,670 patients with 10,545 embryos biopsied overall and 3,611 embryos with ≥1 mosaic chromosome met inclusion criteria The number of embryos biopsied decreased with maternal age (p<0 01) as expected 3,366 (93 2%) embryos had only autosomal chromosome mosaics, which was independent of maternal age (p=0 05) Alternatively, the percent of embryos with ≥1 sex chromosome mosaic (6 8% n=245) was significantly associated with maternal age without clear trend by age group (p<0 01) Table 1 shows PGT-A results by type of mosaicism stratified by maternal age Segmental mosaicism peaked at maternal age 35-37, while complex mosaicism increased with maternal age Full chromosome mosaicism was similar across age groups Conclusions: Among our embryo cohort, rates of segmental mosaicism varied and complex mosaicism increased with maternal age These results remained significant when controlling for paternal age The rate of sex chromosome mosaicism was associated with maternal age but may not be sufficiently powered given the low number of chromosomes Our results provide further data for counseling patients about mosaic embryo results [Formula presented] References: 1 Â An Analysis Of The Effect Of Maternal And Paternal Age On Chromosomal Mosaicism, Pacific Coast Reproductive Society Annual Conference – Cancelled by COVID-19
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