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?:abstract
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Bio-affinity chromatography is used in the study of drug-receptor interactions. A stepwise frontal analysis (SFA) method was developed based on frontal analysis (FA). A high expression alpha 1A adrenergic receptor (α1A AR) cell membrane chromatography (CMC) method was then developed and combined with SFA to investigate the affinity of three model α1A AR-binding drugs towards α1A AR. Equilibrium dissociation constant (Kd) values for drug-receptor interactions were determined by FA and SFA; results showed that these methods were highly consistent. The results demonstrate that the CMC/SFA method is a time-saving and less wasteful method than traditional method for the evaluation of drug-receptor binding characteristics, and could be used to study the interactions between drugs and membrane receptors.
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?:doi
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?:doi
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10.1016/j.jchromb.2020.122436
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?:journal
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Journal_of_chromatography._B,_Analytical_technologies_in_the_biomedical_and_life_sciences
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?:title
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Application of a stepwise frontal analysis method in cell membrane chromatography.
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