| Property | Value |
|---|
|
?:abstract
|
-
Objectives: Fast and precise detection of SARS-CoV-2 RNA in infected patients is essential for treatment decisions Methods: A diagnostic strategy by analyzing nasopharyngeal swabs to detect SARS-CoV-2 RNA in individuals was established The negative impacts of the individual buffer components on RT-qPCR analysis was reviewed and overcome by RNA purification To investigate the functionality of the improved protocol we compared the novel diagnostic strategy to a Bead-based RNA extraction method using previously positive tested samples Results: A method to extract purify RNA molecules from SARS-CoV-2 was established We examined the significance of nucleic acid purification and the need for an RNase inhibitor Evaluation of 3,664 samples from March 23rd until May 18th in 2020 showed the incidence of COVID-19 infections in Thuringia, Germany Additionally, benefits and limits of three RT-qPCR kits were assessed Conclusions: Our study suggests that virus capsid lysis combined with nucleic acid purification enables a viable alternative for the molecular diagnostics of SARS-CoV-2 infections Due to the current delivery delays from different companies, this method offers the possibility to continue diagnosis and to handle the large number of samples
|
|
is
?:annotates
of
|
|
|
?:creator
|
|
|
?:journal
|
-
Journal_of_Laboratory_Medicine_/_Laboratoriums_Medizin
|
|
?:license
|
|
|
?:publication_isRelatedTo_Disease
|
|
|
?:source
|
|
|
?:title
|
-
Optimization of a molecular diagnostic strategy to verify SARS-CoV-2 infections by RT-qPCR
|
|
?:type
|
|
|
?:who_covidence_id
|
|
|
?:year
|
|
![[This page as RDF]](https://research.tib.eu/covid-19/static/rdf-icon.gif){kind=icon}