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  • serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1); binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2); failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1; if red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
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