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  • immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase; while either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye; many variations of the method have been developed.
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