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  • Tubes that contain the stationary phase and through which the mobile phase flows. These columns are the most important part of the separation process in a chromatography system. Chromatography columns are usually made of stainless steel, glass, or plastic and which may vary from 10 to 200 cm in length and 1 to 50 mm in internal diameter. Some columns can withstand high pressures (typically up to 10,000 psi). In column chromatography, migration of the mobile phase through the stationary phase results in sample components within the mobile phase eluting at different rates, permitting collection of the fractions at different times.
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