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  • Filter photometers that determine the emission of electromagnetic radiation from a substance at one wavelength after the substance has absorbed radiation at another wavelength. These instruments usually determine the presence of fluorescent compounds by placing the sample in a beam of light (most frequently ultraviolet), where fluorescent compounds are excited, emitting visible light. Their principal components are a stable light source (e.g., a mercury vapor lamp), a dispersing filter that removes visible light from the excited beam, a sample cuvette (glass for excited wavelengths above 320 nm, otherwise quartz), a secondary filter that removes scattered light from the beam, and usually a photomultiplier tube as a detector.
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