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  • Catalysis of the second transesterification reaction of spliceosomal mRNA splicing. Ligation of the two exons occurs via a transesterification reaction where the free 3\'-hydroxyl group of the 5\' exon is the nucleophile attacking the 3\' splice site. Non-expressed sequences are now detached from the exons. In cis splicing, the intron is in a lariat structure. [GOC:krc, ISBN:0879695897]
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