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  • The process of coupling proline to prolyl-tRNA in a mitochondrion, catalyzed by prolyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2\'- or the 3\'-hydroxyl group of the 3\'-adenosine residue of the tRNA. [GOC:mah, GOC:mcc]
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