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  • A modification of the standard polymerase chain reaction that increases the ability to preferentially amplify minority alleles and somatic mutations in the presence of an excess of wild-type molecules. Sequence-variant molecules are preferentially selected by the lower melting temperature of their mismatched heteroduplex after they\'ve annealed to a wild-type molecule that is present in excess. By carefully selecting the denaturing temperature below the Tm for the wild-type molecule, only the mismatch heteroduplexes will melt and act as efficient templates for strand synthesis.
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