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  • Hematology reagents used to determine the intrinsic clotting activity of the whole blood (i.e., activated clotting time [ACT]). The test is usually performed by adding the whole blood to a test tube or cartridge containing a surface activator. The formation of a clot in a tube that is kept at normal body temperature (about 37 degrees Celsius/98.5 degrees Fahrenheit) determines the ACT value which depends on the activator. Both automated point-of-care analyzers and, less frequently, manual procedures are used to perform the test. ACT coagulation tests are intended to monitor the effect and/or to regulate the administration of high-dose unfractionated heparin before, during, and shortly after procedures (usually surgical) that require intense anticoagulant administration. Dedicated ACT tests using particular reagent activators (e.g., Celite, kaolin, glass) are available.
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