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Immunohematology reagents used in ABO blood grouping that consist of red blood cells of known A and B reactivity. Serum from a person of an unknown group is reacted with red cells of known A and B reactivity. Cells with A antigens are agglutinated by serum of group B persons and cells with B antigens are agglutinated by serum of group A persons, while serum of group AB persons fails to agglutinate both A and B cells. Serum of type O agglutinates both groups of cells. These cells may be used either in manual or automated tests as reversed (confirmatory) tests.
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