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Immunoassay reagents intended to perform qualitative and/or quantitative analyses of a sample of body fluids (typically serum) to determine the hormone thyroxin (3,5,3\',5\'-L- tetraiodothyronine), commonly known as T4, the primary hormone secreted by normal thyroid glands. These assays determine the total circulating T4 in blood, an equilibrium mixture of protein bound and free (typically 0.03% of total T4) hormone, either using isotopic or nonisotopic immunoassays. Determination of T4 is a useful test to assess thyroid hormone production, but the level of T4 may be abnormal without any thyroid disease because of changes in serum binding proteins.
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