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Microscopes with a light source delivered through an array of pinholes embedded in spinning disks. Each pinhole acts as a like a traditional confocal light source--blocking light from outside the focal plane from reaching the detector, thereby, allowing imaging of thin sections from within a thick sample. In spinning disk confocal microscopes each pinhole acts in parallel, imaging multiple locations within the sample simultaneously. The pinholes are arrayed in such a way that spinning the disk during image acquisition allows the entire field of view to be imaged in real time. Spinning disk confocal units are typically mounted on standard conventional or specially designed light microscopes (e.g., upright, inverted stage).The image data is detected, amplified, and usually digitized and displayed on a high-resolution monitor (and/or stored in the memory of a computer). These microscopes are typically used in cell and molecular biology experiments to perform in vivo imaging of fluorescently labeled molecules.
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