PropertyValue
?:abstract
  • INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil.
is ?:annotates of
?:creator
?:doi
?:doi
  • 10.1590/0037-8682-0657-2020
?:journal
  • Revista_da_Sociedade_Brasileira_de_Medicina_Tropical
?:license
  • cc-by
?:pdf_json_files
  • document_parses/pdf_json/f01588c8601576c1974236f8528f9d9b5317ff6e.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7723368.xml.json
?:pmcid
?:pmid
?:pmid
  • 33263691.0
?:publication_isRelatedTo_Disease
?:sha_id
?:source
  • Medline; PMC
?:title
  • In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil
?:type
?:year
  • 2020-11-25

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