PropertyValue
?:abstract
  • Protection against SARS-CoV-2 and SARS-related zoonotic coronaviruses with pandemic potential is urgently needed. To evaluate immunization strategies, we made nanoparticles displaying the receptor-binding domain (RBD) of only SARS-CoV-2 (homotypic nanoparticles) or co-displaying the SARS-CoV-2 RBD along with RBDs from animal betacoronaviruses that represent threats to humans (mosaic nanoparticles; 4–8 distinct RBDs). Mice immunized with RBD-nanoparticles, but not soluble antigen, elicited cross-reactive antibody binding and neutralization responses, confirming increased immunogenicity from multimerization. Mosaic-RBD-nanoparticles elicited antibodies with superior cross-reactive recognition of heterologous RBDs compared to sera from immunizations with homotypic SARS-CoV-2-RBD-nanoparticles or antibodies from COVID-19 convalescent human plasmas. Moreover, sera from mosaic-RBD-immunized mice neutralized heterologous pseudotyped coronaviruses equivalently or better after priming than sera from homotypic SARS-CoV-2-RBD-nanoparticle immunizations, demonstrating no loss of immunogenicity against any particular RBD resulting from co-display. Thus, a single immunization with mosaic-RBD-nanoparticles provides a potential strategy to simultaneously protect against SARS-CoV-2 and emerging zoonotic coronaviruses.
is ?:annotates of
?:creator
?:doi
?:doi
  • 10.1101/2020.11.17.387092
?:journal
  • bioRxiv
?:license
  • cc-by-nc-nd
?:pdf_json_files
  • document_parses/pdf_json/4fecf488126707ad963346a47be7021c4a47d10c.json; document_parses/pdf_json/a128067fdbfb3b41409b97910fdfa54a87ce4afe.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7685334.xml.json
?:pmcid
?:pmid
?:pmid
  • 33236016.0
?:publication_isRelatedTo_Disease
is ?:relation_isRelatedTo_publication of
?:sha_id
?:source
  • BioRxiv; Medline; PMC; WHO
?:title
  • Mosaic RBD nanoparticles elicit neutralizing antibodies against SARS-CoV-2 and zoonotic coronaviruses
?:type
?:year
  • 2020-11-17

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