PropertyValue
?:abstract
  • We developed a de novo protein design strategy to swiftly engineer decoys for neutralizing pathogens that exploit extracellular host proteins to infect the cell Our pipeline allowed the design, validation, and optimization of de novo hACE2 decoys to neutralize SARS-CoV-2 The best decoy, CTC-445 2, binds with low nanomolar affinity and high specificity to the RBD of the spike protein Cryo-EM shows that the design is accurate and can simultaneously bind to all three RBDs of a single spike protein Because the decoy replicates the spike protein target interface in hACE2, it is intrinsically resilient to viral mutational escape A bivalent decoy, CTC-445 2d, shows ~10-fold improvement in binding CTC-445 2d potently neutralizes SARS-CoV-2 infection of cells in vitro and a single intranasal prophylactic dose of decoy protected Syrian hamsters from a subsequent lethal SARS-CoV-2 challenge
is ?:annotates of
?:creator
?:journal
  • Science
?:license
  • unk
?:publication_isRelatedTo_Disease
is ?:relation_isRelatedTo_publication of
?:source
  • WHO
?:title
  • De novo design of potent and resilient hACE2 decoys to neutralize SARS-CoV-2
?:type
?:who_covidence_id
  • #913668
?:year
  • 2020

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