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?:abstract
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This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.
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?:creator
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?:doi
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10.1007/s10096-020-04071-8
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?:doi
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?:journal
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Eur_J_Clin_Microbiol_Infect_Dis
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?:license
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?:pdf_json_files
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document_parses/pdf_json/1ba7dfbcfced8fcca64af036030d56f9619e2f9a.json
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document_parses/pmc_json/PMC7561432.xml.json
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?:pmid
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?:pmid
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?:publication_isRelatedTo_Disease
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?:sha_id
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?:source
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?:title
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Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
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?:type
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?:year
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