PropertyValue
?:abstract
  • This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.
?:creator
?:doi
  • 10.1007/s10096-020-04071-8
?:doi
?:journal
  • Eur_J_Clin_Microbiol_Infect_Dis
?:license
  • no-cc
?:pdf_json_files
  • document_parses/pdf_json/1ba7dfbcfced8fcca64af036030d56f9619e2f9a.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7561432.xml.json
?:pmcid
?:pmid
?:pmid
  • 33063233.0
?:publication_isRelatedTo_Disease
?:sha_id
?:source
  • Medline; PMC
?:title
  • Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
?:type
?:year
  • 2020-10-16

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