PropertyValue
?:abstract
  • Here we provide technical data for amplifying the complete genome of SARS-CoV-2 from clinical samples using only seventeen pairs of primers. We demonstrate that the [C]V2000bp primer panel successfully produces genomes when used with the residual total RNA extracts from positive clinical samples following diagnostic RT-PCRs (with Ct in the range from 13 to 20). The library preparation method reported here includes genome amplification of ~1750-2000 bp fragments followed by ultrasonic fragmentation combined with the introduction of Illumina compatible adapters. Using the SCV2000bp panel, 25 complete SARS-CoV-2 virus genome sequences were sequenced from clinical samples of COVID-19 patients from Moscow obtained in late March - early April.
is ?:annotates of
?:creator
?:doi
?:doi
  • 10.1101/2020.08.04.234880
?:externalLink
?:journal
  • bioRxiv
?:license
  • cc-by-nc-nd
?:publication_isRelatedTo_Disease
?:source
  • BioRxiv; WHO
?:title
  • SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing
?:type
?:who_covidence_id
  • #234880
?:year
  • 2020-08-04

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