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Introduction: SARS-CoV-2 has crossed the species barrier to infect human It is a rapidly spreading virus that has poses a significant public threat and is a considerable burden on the global economy and human health Objective: We characterized the nucleocapsid phosphoprotein (N), membrane protein (M), and envelope protein (E) genes of Indonesian isolates to investigate genetic composition, predict B-cell epitopes, and construct a molecular phylogenetic tree Methods: In the present work, we retrieved the sequences of 16 Indonesian isolates from the GISAID EpiCoV and the Wuhan-Hu-1 isolate (reference sequence) from GenBank, NCBI We used MEGA X to identify mutations in the three structural protein genes and to construct a molecular phylogenetic tree The IEDB was employed to reveal the linear B-cell epitopes and other parameters Allergenicity prediction was evaluated using AllerTOP and ToxinPred was performed to analyze non-toxic antigen prediction Results: In this study, we report the genetic composition of three structural protein genes in Indonesian SARS-CoV-2 isolates Furthermore, we identified the peptide RRGPEQTQGNFGDQELIRQGTDYK from nucleocapsid phosphoprotein to generate a peptide-based vaccine contrary to SARS-CoV-2 Conclusion: In summary, we propose a candidate for a peptide-based vaccine contrary to SARS-CoV-2 However, advance trials such as in vitro and in vivo testing are involved for validation © 2020 EManuscript Technologies All rights reserved
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