|
?:abstract
|
-
To understand the infection of Porcine Deltacoronavirus (PDCoV) in herds with diarrhea, a pair of RT-PCR detection primers were designed based on the conserved sequence of PDCoV M gene The detection limit of RT-PCR is 3 92 10 sup 3 /sup copies / muL, and the specificity and sensitivity are both good This method was used to detect 518 swine diarrhea stool samples from 2015 to 2017 in pig farms around Shanghai, and 25 PDCoV positive samples were detected Among the positive samples detected, the mixed infection rate of PDCoV with porcine kobuvirus (PKV) and porcine astrovirus (PAstV) was higher, at 40% and 48%, respectively;PDCoV and swine epidemic The mixed infection rate of porcine epidemic diarrhea virus (PEDV) was 8 0%, and no mixed samples of PDCoV and porcine transmissible gastroenteritis virus (TEGV) were detected Randomly select 10 PDCoV positive samples and analyze the homology of the amplified M gene fragments As a result, the nucleotide homology in the 10 samples reached 95 0%-99 6%, and the homology with the PDCoV strain registered in GenBank It is 96 1%-100%, indicating that the genetic differences of the current PDCoV strains are not large This study provides a reference for understanding the prevalence of PDCoV
|
![[This page as RDF]](https://research.tib.eu/covid-19/static/rdf-icon.gif){kind=icon}