PropertyValue
?:abstract
  • One goal among microbial ecology researchers is to capture the maximum amount of information from all organisms in a sample. The recent COVID-19 pandemic, caused by the RNA virus SARS-CoV-2, has highlighted a gap in traditional DNA-based protocols, including the high-throughput methods we previously established as field standards. To enable simultaneous SARS-CoV-2 and microbial community profiling, we compare the relative performance of two total nucleic acid extraction protocols and our previously benchmarked protocol. We included a diverse panel of environmental and host-associated sample types, including body sites commonly swabbed for COVID-19 testing. Here we present results comparing the cost, processing time, DNA and RNA yield, microbial community composition, limit of detection, and well-to-well contamination, between these protocols.
is ?:annotates of
?:creator
?:doi
?:doi
  • 10.1101/2020.11.13.370387
?:journal
  • bioRxiv
?:license
  • cc-by-nd
?:pdf_json_files
  • document_parses/pdf_json/ecf62c9a81a42fbb2654f709a1c06945d23d0dae.json; document_parses/pdf_json/7f2d5f3d5a1f8eac246ecf56ccef4a699955c20a.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7668742.xml.json
?:pmcid
?:pmid
?:pmid
  • 33200135.0
?:publication_isRelatedTo_Disease
is ?:relation_isRelatedTo_publication of
?:sha_id
?:source
  • BioRxiv; Medline; PMC; WHO
?:title
  • A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities
?:type
?:year
  • 2020-11-14

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