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?:abstract
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Coronavirus disease (COVID-19), caused by SARS-CoV-2, evolved into a global pandemic in 2020, and the outbreak has taken an enormous toll on individuals, families, communities and societies around the world One practical and effective strategy is to implement rapid case identification based on a rapid testing to respond to this public health crisis Currently, the available technologies used for rapid diagnostics include RT-PCR, RT-LAMP, ELISA and NGS Still, due to their different limitations, they are not well suited for rapid diagnosis in a variety of locations Paper-based devices are alternative approaches to achieve rapid diagnosis, which are cost-effective, highly selective, sensitive, portable, and easy-to-use In addition to individual virus screening, wastewater-based epidemiology has been emerged to be an effective way for early warning of outbreak within the population, which tests genomic viral sequence to reflect information on the spread and distribution of the virus because SARS-CoV-2 can be shed into wastewater through the feces and urine from infected population In this paper, we describe paper-based device as a low-cost and rapid sensor for testing of virus for both diagnosis and early warning of outbreak for wastewater-based epidemiology Most importantly, the device has great potential for real-time detection in the field, without any advanced facilities and well-trained skilled personnel, and provides early warning or timely intervention of an outbreak of pandemic
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