?:abstract
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An outbreak of 2019 novel coronavirus(2019-nCoV), results in novel coronavirus disease that began in Wuhan, China, has spread rapidly with cases now confirmed in multiple countries Nucleic acid amplification test (NAAT), represent by reverse-transcriptase polymerase chain reaction (RT-PCR) plays an important role in disease diagnosis and treatment evaluation The test results by RT-PCR have attracted much attention recently It would be much help to combine the knowledge about its pathogenesis to judge the test results This review will focus on understanding the specific RT-PCR performance of the 2019-nCoV, with combined knowledge of epidemiology, pathogenesis, clinical characteristics and pre-analysis quality control from viral pneumonia
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