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?:abstract
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ABSTRACT: Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. GRAPHIC ABSTRACT: Nucleic acid based detection tool for SARS-CoV-2. [Image: see text]
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?:doi
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10.1007/s11033-020-05889-3
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document_parses/pdf_json/01e5af79d04ddea7b6068898408ad226def3a8c1.json
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document_parses/pmc_json/PMC7538041.xml.json
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?:title
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Recent advances in the nucleic acid-based diagnostic tool for coronavirus
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