kpiq9w36

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?:abstract	Abstract INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil. INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner However, a decrease in the specificity of PCR assays for their targets may lead to false negative results METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences Ten assays had mismatches in up to 3 sequences and two in many sequences CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil.
is ?:annotates of	<https://research.tib.eu/covid-19/entity/kpiq9w36_hasAnnotation_C0017428> <https://research.tib.eu/covid-19/entity/kpiq9w36_hasAnnotation_C0162326> <https://research.tib.eu/covid-19/entity/kpiq9w36_hasAnnotation_C5203676>
?:creator	<https://research.tib.eu/covid-19/entity/Santos%2C_Renan_da_Silva%3B_Bret%2C_Raissa_Souza_Caminha%3B_Moreira%2C_Ana_Cristina_de_Oliveira_Monteiro%3B_Campos%2C_Adriana_Rolim%3B_Silva%2C_Angelo_Roncalli_Alves_E%3B_Lima%2C_Danielle_Malta%3B_Tavares%2C_Kaio_Cesar_Simiano> <https://research.tib.eu/covid-19/entity/Santos%2C_Renan_da_Silva%3B_Bret%2C_Raissa_Souza_Caminha%3B_Moreira%2C_Ana_Cristina_de_Oliveira_Monteiro%3B_Campos%2C_Adriana_Rolim%3B_Silva%2C_Angelo_Roncalli_Alves_e%3B_Lima%2C_Danielle_Malta%3B_Tavares%2C_Kaio_Cesar_Simiano> <https://research.tib.eu/covid-19/entity/Santos%2C_Renan_da_Silva_Bret_Raissa_Souza_Caminha_Moreira_Ana_Cristina_de_Oliveira_Monteiro_Campos_Adriana_Rolim_Silva_Angelo_Roncalli_Alves_e_Lima_Danielle_Malta_Tavares_Kaio_Cesar_Simiano>
?:journal	Rev._Soc._Bras._Med._Trop Rev_Soc_Bras_Med_Trop
?:license	unk
?:publication_isRelatedTo_Disease	<https://research.tib.eu/covid-19/entity/COVID-19>
?:source	WHO
?:title	In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil
?:type	<https://research.tib.eu/covid-19/vocab/Publication>
?:who_covidence_id	#1023055 #1024436 #1043989 #745399 #953373
?:year	2020

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?:abstract

Abstract INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil.
INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner However, a decrease in the specificity of PCR assays for their targets may lead to false negative results METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences Ten assays had mismatches in up to 3 sequences and two in many sequences CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil
INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended PCR assays. RESULTS: Only 3 of the 15 completely aligned to all Brazilian sequences. Ten assays had mismatches in up to 3 sequences and two in many sequences. CONCLUSION: These results should be taken into consideration when using PCR-based diagnostics in Brazil.

is ?:annotates of