PropertyValue
?:abstract
  • A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end point dPCR compared with real-time qPCR, and the limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals. Real-time dPCR showed increased linear dynamic range compared with end point dPCR based on quantitation from amplification curves. Real-time dPCR can improve the performance of TaqMan assays beyond real-time qPCR and end point dPCR with better sensitivity and specificity, absolute quantification and a wider linear range of detection.
is ?:annotates of
?:creator
?:journal
  • Biotechniques
?:license
  • unk
?:publication_isRelatedTo_Disease
?:source
  • WHO
?:title
  • Increased sensitivity using real-time dPCR for detection of SARS-CoV-2
?:type
?:who_covidence_id
  • #940124
?:year
  • 2020

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