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?:abstract
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Modified Vaccinia Ankara (MVA) is a highly attenuated poxvirus vector that is widely used to develop vaccines for infectious diseases and cancer. We demonstrate the construction of a vaccine platform based on a unique three-plasmid system to efficiently generate recombinant MVA vectors from chemically synthesized DNA. In response to the ongoing global pandemic caused by SARS coronavirus-2 (SARS-CoV-2), we use this vaccine platform to rapidly produce fully synthetic MVA (sMVA) vectors co-expressing SARS-CoV-2 spike and nucleocapsid antigens, two immunodominant antigens implicated in protective immunity. We show that mice immunized with these sMVA vectors develop robust SARS-CoV-2 antigen-specific humoral and cellular immune responses, including potent neutralizing antibodies. These results demonstrate the potential of a vaccine platform based on synthetic DNA to efficiently generate recombinant MVA vectors and to rapidly develop a multi-antigenic poxvirus-based SARS-CoV-2 vaccine candidate.
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?:doi
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10.1038/s41467-020-19819-1
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document_parses/pdf_json/e3f684dbf0da1f7244dc5d74facda59f0f96ab27.json; document_parses/pdf_json/107f5f94d943936a28f848643b0400e70868ae06.json
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document_parses/pmc_json/PMC7705736.xml.json
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?:title
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Development of a multi-antigenic SARS-CoV-2 vaccine candidate using a synthetic poxvirus platform
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