PropertyValue
?:abstract
  • In November 2017, a severe infectious disease that devastated the major goose-producing regions in China was found to be caused by a novel goose astrovirus (N-AstV). The objective of this study was to develop a quantitative loop-mediated isothermal amplification (qLAMP) assay for the rapid diagnosis of N-AstV characterized with gout, hemorrhage, and swellings of the kidneys. A set of 4 specific primers, 2 inner and 2 outer primers, targeting the ORF1a gene of N-AstV were designed for the assay which could be completed within 60 min at 65°C in a water bath or on a real-time PCR instrument for quantitative analysis. The qLAMP assay showed a high sensitivity with a detection limit of 1 × 10(1) copies of the target DNA/μL. There were no cross-reactions with other viruses, and the reproducibility of the assay was confirmed in intrasensitivity and intersensitivity assay tests with variability ranging from 0.61 to 2.21%. The results indicated that the qLAMP assay for N-AstV was a simple, accurate, rapid, sensitive, and specific, especially useful for field detection.
is ?:annotates of
?:creator
?:doi
  • 10.1016/j.psj.2020.09.077
?:doi
?:externalLink
?:journal
  • Poult_Sci
?:license
  • cc-by-nc-nd
?:pdf_json_files
  • document_parses/pdf_json/530e488a25362d9351cb33f070ebea97a0edc137.json
?:pmc_json_files
  • document_parses/pmc_json/PMC7705033.xml.json
?:pmcid
?:pmid
?:pmid
  • 33248574
?:publication_isRelatedTo_Disease
is ?:relation_isRelatedTo_publication of
?:sha_id
?:source
  • PMC
?:title
  • A quantitative loop-mediated isothermal amplification assay for detecting a novel goose astrovirus
?:type
?:year
  • 2020-10-08

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